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Answer to: What is the importance of serial dilution? By signing up, you'll get thousands of step-by-step solutions to your homework questions. Diluting is has two primary benefits: It may increase absorption by spreading the oil over a larger surface area It decreases the likelihood of a negative reaction.
A serial dilution is the stepwise of a in. Usually the at each step is constant, resulting in a of the in a fashion. A ten-fold serial dilution could be 1, 0.1 M, 0.01 M, 0.001 M. Serial dilutions are used to accurately create highly diluted solutions as well as solutions for resulting in with a. A tenfold dilution for each step is called a logarithmic dilution or log-dilution, a 3.16-fold (10 0.5-fold) dilution is called a half-logarithmic dilution or half-log dilution, and a 1.78-fold (10 0.25-fold) dilution is called a quarter-logarithmic dilution or quarter-log dilution. Serial dilutions are widely used in experimental sciences, including,. Contents.In biology and medicine In and, besides the more conventional uses described above, serial dilution may also be used to reduce the concentration of microscopic organisms or cells in a sample.
As, for instance, the number and size of that grow on an plate in a given time is concentration-dependent, and since many other diagnostic techniques involve physically counting the number of micro-organisms or cells on specials printed with grids (for comparing concentrations of two organisms or cell types in the sample) or wells of a given volume (for absolute concentrations), dilution can be useful for getting more manageable results. Serial dilution is also a cheaper and simpler method for preparing than. In homeopathy. Experiments in Microbiology, Plant Pathology and Biotechnology. New Age Publishers, 2005, p.
69. Booth, C.; et al. Methods in microbiology 35.
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Academic Press. P. 543. Weissmann, Gerald (2006). The FASEB Journal.
20 (11): 1755–1758. Retrieved 2008-02-01. Ernst, Edzard (November 2005).
'Is homeopathy a clinically valuable approach?' Trends in Pharmacological Sciences. 26 (11): 547–548.
Michael L. Bishop, Edward P. Fody, Larry E. Clinical Chemistry: Principles, Procedures, Correlations.
Lippincott Williams & Wilkins, 2004, p. 24.External links., Bates College.
Ten-fold serial dilutionsA ten-fold dilution reduces the concentration of a solution ora suspension of virus by a factor of ten that is to one-tenth the originalconcentration. A series of ten-fold dilutions is described as ten-fold serialdilutions. In this manual, ten-fold serial dilutions are used in titrations of asuspension of Newcastle disease virus to establish the infectivity titre.
Theyare carried out in small sterile test tubes. These tubes are usually made ofglass and it is preferable if they have fitted lids to minimize the risk ofcontamination during the dilution.A ten-fold dilutionStep 1.Use a micropipette to dispense 900 mL of the diluent to glass tube.Step 2.Use a micropipette to transfer 100 mL of the test solution to the first well. Discard thetip.Step 3.Mix by shaking by hand or using a vortex mixer.Step 4.The well now contains 100 mL ofthe original test solution diluted by one tenth in a total volume of 1000 mL.Ten-fold serial dilutionsStep 1.Set up the sterilized glass test tubes in a rack. Label eachtube clearly to indicate the dilution of its contents after the ten-fold serialdilution has been carried out.Step 2.Use a micropipette to dispense 900 mL of the diluent to all the labeled steriletubes.Step 3.Use a micropipette to transfer 100 mL of the test solution to the first tube and mix. Thisis the first ten-fold dilution.Step 4.Use a micropipette with new sterile tip to carry out a secondten-fold dilution.Step 5.Continue the series of ten-fold dilutions until the lasttube.
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